Quantitative microimmunohistochemistry (QµIC)
Anna Fomitcheva Khartchenko, Aditya Kashyap, et al.
MicroTAS 2018
We have developed a method for spatially resolved genetic analysis of formalin-fixed paraffin-embedded (FFPE) cell block and tissue sections. This method involves local sampling using hydrodynamic flow confinement of a lysis buffer, followed by electrokinetic purification of nucleic acids from the sampled lysate. We characterized the method by locally sampling an array of points with a circa 200 μm diameter footprint, enabling the detection of single KRAS and BRAF point mutations in small populations of RKO and MCF-7 FFPE cell blocks. To illustrate the utility of this approach for genetic analysis, we demonstrate spatially resolved genotyping of FFPE sections of human breast invasive ductal carcinoma.
Anna Fomitcheva Khartchenko, Aditya Kashyap, et al.
MicroTAS 2018
Lena Voith von Voithenberg, Aditya Kashyap, et al.
Small
Aditya Kashyap, Anna Fomitcheva Khartchenko, et al.
Nature Biomedical Engineering
Lena Voith von Voithenberg, Aditya Kashyap, et al.
Small